Label-free DIA-based workflow for single-cell proteomic analysis on an Orbitrap Ascend Tribrid mass spectrometer. — ASN Events
  1. Schedule
  2. Accepted Posters
  3. Label-free DIA-based workflow for single-cell proteomic analysis on an Orbitrap Ascend Tribrid mass spectrometer.

Label-free DIA-based workflow for single-cell proteomic analysis on an Orbitrap Ascend Tribrid mass spectrometer. (#413)

Joanne Ford 1 , Fernanda Salvato 2 , Tonya Pekar Hart 2 , Amirmansoor Hakimi 2 , Delanghe Bernard 3 , Julia Kraegenbring 3
  1. Thermo Fisher Scientific, Scoresby, VIC, Australia
  2. Thermo Fisher Scientific, San Jose, San Jose, California, USA
  3. Thermo Fisher Scientific, GmbH, Bremen, Germany

Purpose: Demonstrate the performance of the Thermo Scientific Orbitrap Ascend Tribrid mass spectrometer (MS) and Thermo Scientific Vanquish Neo UHPLC system in the analysis of low input and single cell samples.

Methods: A label-free DIA-based method that gives a throughput of 50 samples per day (SPD) is demonstrated with low input samples (HeLa peptides) and single cell samples. Results: Our results show that the Orbitrap Ascend Tribrid MS coupled to Vanquish Neo UHPLC has single cell sensitivity.

#ausomics2025